Synonyms:
Macrophage migration inhibitory factor, Glutathione-binding 13 kDa protein, Ldopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase
Description:
Macrophage Migration Inhibitory Factor (MIF) was one of the first cytokine activities to be discovered, initially described as a T cell-derived factor that inhibits the random migration of macrophages. Later, MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. Secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF plays a critical role in the host control of inflammation and immunity.
Uniprot Name:
Macrophage migration inhibitory factor
Specificity:
Rat and mouse MIF
Immunogen:
E.coli-expressed full-length recombinant rat MIF
Formulation:
Lyophilized with 0.1% sodium azide
Purification:
Protein A affinity chromatography
Reconstitution:
Centrifuge vial prior to opening. Add 200 μl sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1.0 mg/ml.
Application:
ELISA, IHC, Neutralizing , WB
Application notes:
Western blot: 1:2000
Immunohistochemistry: 1:500
Storage/Stability:
Store at 2-8°C for short term and in working aliquots at -20°C to -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.
