Synonyms:
MIF, GIF, GLIF, MMIF
Description:
This polyclonal antibody recognizes Macrophage Migration Inhibitory Factor (MIF), one of
the first cytokine activities to be discovered, initially described as a T cell-derived factor
that inhibits the random migration of macrophages. Later, MIF was rediscovered as a
pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action
within the immune system. MIF is released from macrophages and T cells in response to
physiological concentrations of glucocorticoids. Secreted MIF counter-regulates the
immunosuppressive effects of steroids on immune cell activation and cytokine
production. MIF plays a critical role in the host control of inflammation and immunity.
Uniprot Name:
Macrophage migration inhibitory factor
Immunogen:
E.coli-expressed full-length recombinant rat MIF
Formulation:
Lyophilized with 0.05% sodium azide
Purification:
Protein A affinity chromatography
Reconstitution:
Centrifuge vial prior to opening. Add 200 μl sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1.0 mg/ml.
Application:
ELISA, IHC, Neutralizing , WB
Application notes:
Western blot: 1:2000
Immunohistochemistry: 1:500
Storage/Stability:
Store at 2-8 °C short term and in working aliquots at -20 °C for long term storage. Avoid repeated freeze-thaw cycles.
