Synonyms:
Mif, GIF, GLIF, MMIF
Description:
This polyclonal antibody recognizes Macrophage Migration Inhibitory Factor (MIF), one of the first cytokine activities to be discovered, initially described as a T cell-derived factor that inhibits the random migration of macrophages. Later, MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. Secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF plays a critical role in the host control of inflammation and immunity.
Specificity:
Recognizes both natural and recombinant rat MIF
Immunogen:
Recombinant full-length rat MIF, aa 1-115 (full length)
Formulation:
Lyphilized from 1xPBS
Purification:
Protein A Chromatography
Reconstitution:
Centrifuge vial prior to opening. Add 200 μl sterile distilled water to the vial to fully solubilize the antibody to a concentration of 1.0 mg/ml.
Application notes:
Western blot: 1:2000
Immunohistochemistry: 1:500
Storage/Stability:
Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.
