Rabbit Anti-SARS-CoV-2 Spike S1 (Clone 007) mAb

Synonyms:

Spike glycoprotein S1, Receptor binding domain Spike protein, RBD spike protein

Species:

SARS-CoV-2

Cat. No.:

CPC500A pdf (datasheet)

Quantity/Size:

50 μl

Price:

$220.00 BUY

Concentration:

1.0 mg/ml

Description:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID -19). The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. Spike glycoprotein is cleaved into the following 3 chains, Spike protein S1, Spike protein S2, Spike protein S2'. Spike protein S1 attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein. Surface glycoprotein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Uniprot Name:

Spike glycoprotein S1

Uniprot ID:

P0DTC2

Data PDF:

CPC500

Specificity:

2019-nCoV Coronavirus spike protein

Host Species:

Rabbit

Isotype:

IgG

Clone:

007

Formulation:

Sterile-filtered PBS, preservative free.

Purification:

Protein A affinity chromatography

Application:

ELISA, IF

Application notes:

ELISA: suggested dilution 1:5,000 - 1:10,000
Immunofluorescence: suggested dilution 1:60

IHC, FCM, IP applications have not been validated.
Product applications have not been validated with corresponding virus positive samples. Optimal concentrations should be determined by the end user.

Storage/Stability:

Stable at 2-8°C for 1 month or for up to 1 year at -20°C to -80°C. It is recommended to prepare working aliquots of undiluted product and store -20°C to -80°C.