Rabbit Anti-SARS-CoV-2 Nucleocapsid (Clone 019) mAb

Synonyms:

Nucleoprotein, Nucleocapsid protein, NC, NP, Protein N

Species:

SARS-CoV-2

Cat. No.:

CPC501A pdf (datasheet)

Quantity/Size:

50 μl

Price:

$220.00 BUY

Concentration:

1.0 mg/ml

Description:

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Uniprot Name:

Nucleoprotein

Uniprot ID:

P0DTC9

Data PDF:

CPC501

Specificity:

2019-nCoV Coronavirus Nucleocapsid

Host Species:

Rabbit

Isotype:

IgG

Clone:

019

Formulation:

Sterile-filtered PBS, preservative free.

Purification:

Protein A affinity chromatography

Application:

ELISA, WB

Application notes:

ELISA: suggested dilution 1:5,000 - 1:10,000
Western blot: suggested dilution 1:1,000 - 1:5,000

IHC, FCM, IP, IF applications have not been validated.
Product applications have not been validated with corresponding virus positive samples. Optimal concentrations should be determined by the end user.

Storage/Stability:

Stable at 2-8°C for 1 month or for up to 1 year at -20°C to -80°C. It is recommended to prepare working aliquots of undiluted product and store -20°C to -80°C.