Rat Anti-Mouse Pan-endothelial Cell Antigen (Clone MECA-32) mAb

Molecule:

MECA32

Synonyms:

MECA-32 antigen, plasmalemma vesicle protein 1, plasmalemma vesicle-associated protein, Pv1, MECA32

Species:

Mouse

Cat. No.:

CMP027 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$340.00 BUY

Description:

The MECA-32 antibody reacts with a dimer of 50-55–kDa subunits expressed on most or all endothelial cells in the embryonic and adult mouse, with the exception of cardiac and skeletal muscle and the brain.  Typically in skeletal and cardiac muscle, MECA-32 antigen expression is limited to small arterioles and venules; however, under conditions of inflammation, it can be induced on previously non-expressing vessels in cardiac muscle.  In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated.  During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears.  The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation.  In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.

Entrez Symbol:

Plvap

Uniprot Name:

Plasmalemma vesicle-associated protein

Uniprot ID:

Q91VC4

Data PDF:

CMP027

Specificity:

The MECA-32 antibody reacts with the “Plasmalemma vesicle-associated protein”, a dimer of 50-55 kDa subunits expressed on most or all endothelial cells surfaces in the embryonic and adult mouse, with the exception of cardiac and skeletal mu

Host Species:

Rat

Immunogen:

Mouse lymph-node stromal cells

Isotype:

IgG2A

Clone:

MECA-32

Formulation:

PBS, pH 7.4 without preservative and additives.

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 1 mg/ml. The solution can then be further diluted in other aqueous buffers.

Application:

FC, ICC, IHC-F, IP

Application notes:

Wester Blot, FACS, IP, IHC-F, ICC, IF (1 μg/106 cells)

Storage/Stability:

Store at 2-8°C.