Description:
Product: The antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat) immunized with mouse PlGF recombinant protein. The IgG2 fraction of culture supernatant was purified by Protein G affinity chromatography.
Uniprot Name:
Placenta growth factor
Specificity:
Recognizes mouse PlGF in Western blot.
Immunogen:
Mouse PlGF recombinant protein
Formulation:
Lyophilized from a sterile filtered (through a 0.2 �m filter) solution in PBS without additives.
Purification:
Protein G Chromatography
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile PBS to a concentration of 0.2 mg/ml. The solution can then be further diluted in other aqueous buffers.
Application notes:
Western blot: 1:400-1,000 | Immunohistochemistry: 1:50-100. Optimal dilutions should be determined by each laboratory for each application.
Storage/Stability:
Lyophilized antibody is stable at -80℃C for 2 years from date of receipt. After reconstitution, aliquot is stable for ?6 months at <-20℃C. Avoid repeated freeze-thaw cycles.