Rat Factor XII Total Antigen ELISA kit


Factor XII

Cat. No.:

CKR003B pdf (datasheet)


5 x 96 tests


$2,905.00 BUY


Intended Use: This rat coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII antigen in rat plasma. This kit has been formulated for research only.

Background: Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen. Factor XII is activated by kallikrein. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a ‘severe defect’ in thrombus formation.

Assay Principle: Rat Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti-rat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat Factor XII. Color development is proportional to the concentration of Factor XII in the sample.

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Store kit components at 4°C. Store unopened plate and any unused microtiter strips in pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT freeze/thaw the standard & primary Ab more than once.