Rat Spleen Tissue Lysate

Species:

Rat

Cat. No.:

PX420A pdf (datasheet)

Quantity/Size:

100 μg

Price:

$190.00 BUY

Description:

Rat spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The tissue lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

Concentration:

2.0 mg/ml

Data PDF:

PX420

Formulation:

Spleen tissue lysate is supplied in 1 x SDS sample buffer containing 5% bmercaptoethanol.

Storage/Stability:

Store at 2-8°C for continuous use. Store in working aliquots at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.

Application notes:

Centrifuge vial before use. Rat spleen tissue lysate is ready to load on SDS-PAGE for Western blotting, 10-20 µg per lane is recommended for mini gel. The optimal concentration should be determined for each specific application.

Application:

WB