Rat VEGF ELISA Kit

Molecule:

VEGF

Synonyms:

VEGF, Vascular endothelial growth factor, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E

Cat. No.:

CK448 pdf (datasheet)

Quantity/Size:

1 Plate

Price:

$575.00 BUY

Description:

The Cell SciencesĀ® Rat VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat VEGF in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for rat VEGF coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Data PDF:

CK448