Synonyms:
VEGF, Vascular endothelial growth factor, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E
Description:
The Cell Sciences® Rat VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat VEGF in serum, plasma, and cell
culture supernatants. This assay employs an antibody specific for rat VEGF coated on a 96-well plate.
Standards and samples are pipetted into the wells and VEGF present in a sample is bound to the wells by
the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After
washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The
wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion
to the amount of VEGF bound. The Stop Solution changes the color from blue to yellow, and the intensity
of the color is measured at 450 nm.
