Recombinant Cys-Protein G

Synonyms:

Immunoglobulin G-binding protein G

Species:

Streptococcus

Cat. No.:

CS479A pdf (datasheet)

Quantity/Size:

1 mg

Price:

$225.00 BUY

Description:

Protein G binds to the constant region of many species of immunoglobulin G. It can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains 3 IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. Recombinant Cys-Protein G contains 201 amino acid residues of the Streptococcus sp including a Cys residue at N-terminus.

Entrez Symbol:

spg

Uniprot Name:

Immunoglobulin G-binding protein G

Uniprot ID:

P19909

Data PDF:

CS479

Source:

E. coli

Molecular Weight:

Recombinant Cys-Protein G has a predicted molecular mass of approximately 21.9 kDa but migrates with an apparent molecular mass of about 40 kDa in SDS PAGE.

Physical State:

Lyophilized

Formulation:

Lyophilized with no additives

Purity:

>95% by SDS-PAGE and RP-HPLC

Specific Activity:

1. Binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a. 2. Does not bind to human IgM, IgD and IgA.

Endotoxin Level:

< 0.1 EU/µg, determined by LAL method.

Amino Acid Sequence:

CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E

Reconstitution:

Centrifuge vial prior to opening. Dissolve in distilled water or saline.

Storage/Stability:

Store at -20°C to -80°C.