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Recombinant Equine PDGF-BB, Animal Free

Molecule:

PDGFB

Synonyms:

Platelet derived growth factor subunit B

Species:

Horse

Cat. No.:

CRE050A-AF pdf (datasheet)

Quantity/Size:

5 μg

Price:

$95.00 BUY

Description:

Platelet-Derived Growth Factor (PDGF) is a member of the protein family which includes vascular endothelial growth factors (VEGF), important regulators of cell growth, proliferation, and angiogenesis. PDGF is proteolytically processed from a pre/pro protein to generate PDGF subunit B, which can homodimerize, or can heterodimerize with the related PDGF subunit A. The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.
PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets, released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF.

Manufactured without Animal-derived products, in an Animal Free facility.

Entrez Symbol:

PDGFB

Uniprot Name:

Platelet-derived growth factor subunit B

Uniprot ID:

A0A3Q2GVH4

Data PDF:

CRE050-AF

Source:

E. coli

Molecular Weight:

Dimer, 12.4/24.8 kDa (110/220 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered10 mM sodium phosphate, pH 7.5

Purity:

≥95.0% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 30 ng/ml, determined by the dose-dependant proliferation of 3T3 cells.

Specific Activity:

> 3.3 x 10e4 IU/mg, calibrated against the International Standard, NIBSC code: 94/728.

Endotoxin Level:

< 1 EU/µg by kinetic LAL

Amino Acid Sequence:

MSLGSLAVAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRHVQ CRPTQVQLRP VQVRKIEIVR KKPTFKKATV TLEDHLACKC ETVGAARPVT

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.