Recombinant Hirudin

Molecule:

Hirudin

Cat. No.:

CRH117A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$215.00 BUY

Description:

Hirudin is a potent thrombin-specific protease inhibitor originally derived from a freshwater leech. Unlike heparin, hirudin acts directly on thrombin, rather than through other clotting factors. It has a high binding affinity and specificity for thrombin. Therefore, hirundin prevents or dissolves the formation of clots and thrombi and has a therapeutic value in blood coagulation disorders, for the treatment of skin hematomas and of superficial varicose veins, either as an injectable or a topical cream.

Uniprot Name:

Hirudin

Uniprot ID:

P84590

Data PDF:

CRH117

Source:

Pichia pastoris

Molecular Weight:

6.7 kDa (63 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile filter 20 mM PBS, pH 7.0 containing 2% mannitol.

Purity:

> 96% by SDS-PAGE and HPLC

Biological Activity:

The biological activity is measured by chromogenic assay. 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin.

Specific Activity:

≥14,000 ATU/mg protein.

Endotoxin Level:

<1 EU/mg

Amino Acid Sequence:

VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Further dilution should be made in appropriate buffered solutions.

Storage/Stability:

Store at -20°C to -80 °C for up to one year. Upon reconstitution, product is stable at 2-8 ° C for one month. For longer term, store in working aliquots containing 0.1% BSA at -20° C to -80°C. Avoid repeated freeze-thaw cycles.