Recombinant Human Artemin

Synonyms:

Enovin, neublastin, ARTN

Species:

Human

Cat. No.:

CRA001A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$85.00 BUY

Description:

Artemin is a member of the glial cell line-derived neurotrophic factor (GNDF) ligand family. Artemin is a ligand for the receptor complex, GFRalpha3-RET, as well as activates the GFRalpha1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue.

Entrez Symbol:

ARTN

Uniprot ID:

Q5T4W7

Data PDF:

CRA001

Source:

E. coli

Molecular Weight:

12.0/23.9 kDa (113/226 aa) Non-glycosylated, disulfide-linked homodimer

Physical State:

Lyophilized

Formulation:

Sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium citrate, 25 mM sodium chloride, pH 4.5

Purity:

≥95%, determined by reducing and non-reducing SDS-PAGE

Biological Activity:

The activity is determined by the dose-dependant proliferation of the SH-SY5Y cell line and is typically 4-8 ng/mL. The activity can also be determined by its ability to promote survival and neurite outgrowth.

Endotoxin Level:

≤ 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG

Reconstitution:

Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.