Recombinant Human BAFF

Molecule:

BAFF

Synonyms:

B lymphocyte stimulator, BLyS, B cell activating factor, BAFF, CD257, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1,

Species:

Human

Cat. No.:

CRB300C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$5,680.00 / Special Price: $3,720.00 BUY

Description:

B cell activating facotr is a TNF ligand superfamily member and has been designated TNFSF13B. Produced by macrophages, dendritic cells, and T lymphocytes, BAFF promotes the survival of B cells and is essential for B cell maturation. BAFF binds to three TNF receptor superfamily members: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF R selectively binds BAFF. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes.

Entrez Symbol:

TNFSF13B

Uniprot Name:

Tumor necrosis factor ligand superfamily member 13B

Uniprot ID:

Q9Y275

Data PDF:

CRB300

Source:

E. coli

Molecular Weight:

Monomer, 18.5 kDa (163 aa)

Physical State:

Lyophilized

Formulation:

Sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Purity:

>90%, determined by reducing and non-reducing SDS-PAGE

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using anti-IgM stimulated mouse B cells is less than 2 ng/ml.

Specific Activity:

>5x10E5 IU/mg in the presence of goat anti-mouse IgM

Endotoxin Level:

< 1EU/μg, determined by kinetic LAL

Amino Acid Sequence:

MHHHHHHLVP RAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.