Recombinant Human Beta Defensin 3

Molecule:

BD-3

Synonyms:

Beta-defensin 103, BD-3, DEFB-3, hBD-3, HBD3, HBP-3, Defensin, beta 103

Species:

Human

Cat. No.:

CRB502A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$85.00 BUY

Description:

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alphadefensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the Cterminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues.

Entrez Symbol:

DEFB103A

Uniprot Name:

Beta-defensin 103

Uniprot ID:

P81534

Data PDF:

CRB502

Source:

E. coli

Molecular Weight:

5.2 kDa (45 aa) monomer

Formulation:

Lyophilized from a sterile-filtered solution containing 0.1% Trifluoroacetic acid (TFA)

Purity:

≥ 95% by Reducing and Non-reducing SDS-PAGE

Endotoxin Level:

≤ 1 EU/μg as determined by kinetic LAL method

Amino Acid Sequence:

GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.