Synonyms:
Abrineurin, BDNF, ANON2, BULN2
Description:
Recombinant human BDNF is a non-glycosylated homodimer, containing two 119 amino acid chains, with a total molecular weight of 27 kDa. Background: Brain Derived Neurotrophic Factor, or BDNF, is a nerve growth factor that supports neuron growth and survival. BDNF shares identical domains with two other neurotrophic factors known as, β-NGF and NT-3 (neurotrophin-3). BDNF binds with low affinity to a receptor known as LNGFR, which also binds NGF and NT-3, but mediates survival function by signaling through a high affinity receptor known as gp145/TrkB. Human, mouse, rat and pig BDNF are all cross-reactive.
Uniprot Name:
Brain-derived neurotrophic factor
Genbank Accession No:
P23560
Molecular Weight:
~27 kDa (dimer)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
≥95.0% by SDS-PAGE
Biological Activity:
The ED50 as determined by the dose-dependent proliferation of C6 cells is typically between 0.5-1.5 μg/mL
Specific Activity:
1 x 10E3 units/mg
Endotoxin Level:
≤1 U/µg of protein by kinetic LAL analysis (50% confidence range).
Amino Acid Sequence:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
