Synonyms:
BDNF, Abrineurin, ANON2, BULN2
Description:
Brain Derived Neurotrophic Factor, or BDNF, is a nerve growth factor that supports
neuron growth and survival. BDNF shares identical domains with two other neurotrophic
factors known as, β-NGF and NT-3 (neurotrophin-3). BDNF binds with low affinity to a
receptor known as LNGFR, which also binds NGF and NT-3, but mediates survival
function by signaling through a high affinity receptor known as gp145/TrkB. Human,
mouse, rat and pig BDNF are all cross-reactive.
Uniprot Name:
Brain-derived neurotrophic factor
Genbank Accession No:
P23560
Molecular Weight:
Homodimer (non-covalent), 13.6/27.3 kDa (120/240 aa)
Formulation:
Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
> 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤2 ug/ml, determined by the dose dependent proliferation of C6 cells.
Specific Activity:
≥5.0 x 102 U/mg
Endotoxin Level:
≤ 1 EU/µg by kinetic LAL
Amino Acid Sequence:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a recommended concentration of 0.1 mg/mL and gentely pipet solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
