Recombinant Human BCA-1 / CXCL13

Molecule:

CXCL13

Synonyms:

C-X-C motif chemokine 13, B cell-attracting chemokine 1, BCA-1, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13

Species:

Human

Cat. No.:

CRB400C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,720.00 / Special Price: $2,220.00 BUY

Description:

BCA-1/CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23 - 34% amino acid sequence identity with other known CXC chemokines. Recombinant BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

Entrez Symbol:

CXCL13

Uniprot Name:

C-X-C motif chemokine 13

Uniprot ID:

O43927

Data PDF:

CRB400

Source:

E. coli

Molecular Weight:

10.2 kDa (86 aa) monomer

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 1% Trifluoroacetic acid

Purity:

≥ 95% as determined by reducing and non-reducing SDS-PAGE

Endotoxin Level:

≤ 1 EU/μg as determined by LAL method

Amino Acid Sequence:

VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilution should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.