Recombinant Human BMP-4 active

Molecule:

BMP-4

Synonyms:

Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B

Species:

Human

Cat. No.:

CRB137B pdf (datasheet)

Quantity/Size:

50 μg

Price:

$825.00 BUY

Description:

BMPs (bone morphogenetic proteins) belong to the TGF-β superfamily of structurally related signaling proteins. As implied by their name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. Recombinant human BMP-4 is a 13 kDa monomeric, non-glycosylated, polypeptide chain containing 116 amino acid residues.

Uniprot Name:

Bone morphogenetic protein 4

Uniprot ID:

P12644

Source:

E. coli

Molecular Weight:

13 kDa (116 aa) monomer

Physical State:

Sterile filtered and lyophilized

Formulation:

Lyophilized from sterile-filtered PBS

Purity:

>98% by SDS-PAGE and HPLC analyses

Biological Activity:

ED50 = 2-12 µg/ml, as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells.

Endotoxin Level:

< 0.1 ng/µg of BMP-4

Reconstitution:

Centrifuge vial prior to opening. Reconstitute to a concentration of 0.1-1.0 mg/ml in sterile 20 mM acetic acid. This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use.

Storage/Stability:

The lyophilized protein is stable at -20°C to -80°C for up to 1 year. Reconstituted BMP-4 should be stored in working aliquots at -20°C to -80°C. It is recommended to add a carrier protein (0.1 BSA). Avoid repeated freeze-thaw cycles.