Synonyms:
BMPR1A, Bone morphogenetic protein receptor type-1A, BMPR-1A, Activin receptor-like kinase 3, ALK-3, CD292, Serine/threonine-protein kinase receptor R5, SKR5
Cat. No.:
CRB703-100
pdf (datasheet)
Description:
Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be
mediated by the formation of hetero-oligomeric complexes of the type I and type II
serine/threonine kinase receptors. BMPR1A is one of seven known type I
serine/threonine kinases that are required for the signal transduction of the TGF-b family
cytokines. In contrast to the TGF-b receptor system in which the type I receptor does not
bind TGF-b in the absence of the type II receptor, type I receptors involved in BMP
signaling (including BMPR1A, BMPR1B/ALK6, and ActR-I/ALK2) can independently bind
the various BMP family proteins in the absence of type II receptors.
Recombinant soluble BMPR1A binds BMP2 and -4 with high-affinity in solution and is a
potent BMP2/4 antagonist in vitro. BMPR1A is ubiquitously expressed during
embryogenesis. In adult tissues, BMPR1A mRNA is also widely distributed, with the
highest expression levels found in skeletal muscle. The extracellular domain of BMPR1A
shares little amino acid sequence identity with the other mammalian ALK type I receptor
kinases, but the cysteine residues are conserved. Human and mouse BMPR1A are
highly conserved and share 98% sequence identity.
Molecular Weight:
23 kDa monomer (135 aa)
Formulation:
Lyophilized from a sterile filtered solution containing PBS
Purity:
> 90% determined by SDS-PAGE visualized with silver stain
Biological Activity:
ED50 is typically 1-3 µg/ml, determined by the ability to inhibit recombinant human BMP2- induced (500 ng/nl) alkaline phosphatase production by C2C12 myogenic cells
Endotoxin Level:
< 1 EU/µg
Storage/Stability:
Stable at -20°C to -80° for up to 1 year
