Recombinant Human CCL11/Eotaxin

Molecule:

Eotaxin

Synonyms:

SCYA11, eotaxin-1, C-C motif chemokine 11, small-inducible cytokine A11, eosinophil chemotactic protein, small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Species:

Human

Cat. No.:

CRE000C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 BUY

Description:

Recombinant human CCL11 is a single, non-glycosylated polypeptide chain containing 74 amino acids.

Eotaxin/CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor. CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates.

Entrez Symbol:

CCL11

Uniprot Name:

Eotaxin

Uniprot ID:

P51671

Source:

E. coli

Molecular Weight:

8.3 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 µm sterile filtered solution in in 20 mM PB, pH 7.4 + 150 mM NaCl

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human peripheral blood eosinophils is less than 10 ng/ml.

Specific Activity:

>1.0 × 10E5

Endotoxin Level:

<1EU/�g

Amino Acid Sequence:

GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be further diluted in other aqueous buffers.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage.