Recombinant Human MIP-1 alpha / CCL3

Molecule:

CCL3

Synonyms:

Macrophage Inflammatory Protein 1-alpha, CCL3, LD78-alpha, MIP1A, MIP-1-alpha, SCI, SCYA3, SIS-beta, TY5

Species:

Human

Cat. No.:

CRM400A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$190.00 BUY

Description:

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related by having the four highly conserved cysteine residues present in CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells.

Entrez Symbol:

CCL3

Uniprot Name:

C-C motif chemokine 3

Uniprot ID:

P10147

Data PDF:

CRM400

Source:

E. coli

Molecular Weight:

~7.8 kDa (70 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile-filtered 20 mM PB, pH 7.4, containing 100 mM NaCl.

Purity:

>96% as determined by HPLC and SDS-PAGE analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Endotoxin Level:

<1 EU/μg of recombinant human MIP-1α/CCL3 as determined by LAL method.

Amino Acid Sequence:

ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Upon receipt, store at -20°C to -80°C for up to 1 year. After reconstitution, the preparation is stable for up to one week at 2-8°C or store in aliquots at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.