Recombinant Human CD14

Molecule:

CD14

Synonyms:

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen

Species:

Human

Cat. No.:

CRCC01 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$275.00 BUY

Description:

CD14 acts as a receptor for endotoxin (LPS) and is expressed on monocytes, macrophages, and neutrophils. CD14 is anchored to cells by glycosylphosphatidylinositol (GPI)-linkage and functions as a high affinity receptor of LPS-LBP-complexes. Together with TLR4 and a lymphocytic antigen (MD2) CD14 is the lipopolysaccharide (LPS)-Receptor and mediates innate immune response to bacterial lipoproteins. In endothelial cells CD14 has a regulating function for cell survival. In the presence of LPS CD14 expression can be amplified by TNF-α and IL-1β (in varied tissues differently). Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids which is directly secreted or derived from protease-dependent shedding of the membrane bound molecule. Soluble CD14 (sCD14) competes with membrane bound CD14 (mCD14) for LPS binding and is able to neutralize LPS-induced responses in vitro and in vivo and mediates the LPS-induced activation of non-CD14 expressing endothelial, epithelial and smooth muscle cells.

Concentration:

1 mg/ml prior to lyophilization.

Entrez Symbol:

CD14

Uniprot Name:

Monocyte differentiation antigen CD14

Uniprot ID:

P08571

Data PDF:

CRCC01

Source:

CHO cells

Molecular Weight:

50 kDa

Physical State:

Lyophilized

Formulation:

PBS, pH 7.2.

Purity:

90-95% by SDS-PAGE analysis.

Biological Activity:

Up to 10 µg/mL CD14 inhibits binding of FITC-LPS (0.5 µg/mL) to 6 x 105 CD14 CHO cell transfectants as determined by flow cytometry.

Endotoxin Level:

<0.01 ng/ml

Reconstitution:

Centrifuge vial prior to opening. Add 10 µL sterile distilled water to the vial. Further dilution can be made with PBS or other buffers.

Storage/Stability:

Store as supplied at -80°C for up to 1 year.

Application:

ELISA, FC, INH