Recombinant Human CD14 Cell Culture Supernatant

Molecule:

CD14

Synonyms:

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen

Species:

Human

Cat. No.:

CRCC02 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$350.00 BUY

Description:

CD14 acts as a receptor for endotoxin (LPS) and is expressed on monocytes, macrophages, and neutrophils. CD14 is anchored to cells by glycosylphosphatidylinositol (GPI)-linkage and functions as a high affinity receptor of LPS-LBP-complexes. Together with TLR4 and a lymphocytic antigen (MD2) CD14 is the lipopolysaccharide (LPS)-Receptor and mediates innate immune response to bacterial lipoproteins. In endothelial cells CD14 has a regulating function for cell survival. In the presence of LPS, CD14 expression can be amplified by TNF-α and IL-1β (in varied tissues differently). Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids which is directly secreted or derived from protease-dependent shedding of the membrane bound molecule. Soluble CD14 (sCD14) competes with membrane bound CD14 (mCD14) for LPS binding and is able to neutralize LPS-induced responses in vitro and in vivo and mediates the LPS-induced activation of non-CD14 expressing endothelial, epithelial and smooth muscle cells.

Entrez Symbol:

CD14

Uniprot Name:

Monocyte differentiation antigen CD14

Uniprot ID:

P08571

Data PDF:

CRCC02

Source:

CHO cells

Molecular Weight:

50 kDa

Physical State:

Lyophilized

Formulation:

CHO-S-SFM-II(Gibco), antibiotics, without NaN3

Biological Activity:

The transfected cells were cultivated in a mini-reactor Characteristic: 50μg/ml totally inhibits binding of FITC-LP(0.5μg/ml) to 6 x 105 CD14+CHO transfectants. Up to 10μg/ml inhibits binding of LPS-FITC.

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in 10 �l sterile ddH2O. The solution can then be further diluted in other aqueous buffers.

Application notes:

Product is unpurified cell culture supernatant. For special tests (ex. Cell culture experiments) a control (cell culture supernatant of vector transfected CHO cells, Cat. No. CRS150) is necessary.

Application:

FC, INH