Recombinant Human CX3CL1/Fractalkine

Molecule:

CX3CL1

Synonyms:

Fractalkine, Neurotactin, CXC3, CXC3C, NTN, NTT, SCYD1

Species:

Human

Cat. No.:

CRF200C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,720.00 / Special Price: $2,100.00 BUY

Description:

Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique CX3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human fractalkine cDNA encodes a 397 amino acid (a.a.) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human fractalkine can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble fractalkine. The soluble chemokine domain of human fractalkine was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse fractalkine was reported to chemoattract neutrophils and T-lympho-cytes but not monocytes.

Uniprot ID:

P78423

Data PDF:

CRF200

Source:

E. coli

Molecular Weight:

8.6 kDa (76 aa)

Formulation:

Lyophilized from a sterile-filtered solution containing 0.1% Trifluoroacetic Acid (TFA)

Purity:

> 95% as determined by reducing and non-reducing SDS-PAGE

Biological Activity:

Biological activity determined by human PBMC chemotaxis (first detectable at 10-100 ng/mL). No acceptance criteria established.

Endotoxin Level:

< 1EU/μg as determined by kinetic LAL analysis.

Amino Acid Sequence:

QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.