Synonyms:
IL-8, C-X-C motif chemokine 8, T-cell chemotactic factor
Description:
Interleukin-8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocytederived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (C-X-C) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.
Uniprot Name:
Interleukin-8
Molecular Weight:
8.9 kDa (77 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Purity:
> 95.0% by reducing and non-reducing SDS-PAGE
Biological Activity:
Biological activity is demonstrated starting at 10 ng/ml by Human neutrophil chemotaxis, and starting at 1 ng/ml for Human neutrophil degranulation.
Endotoxin Level:
< 1 EU/μg as determined by kinetic LAL analysis.
Amino Acid Sequence:
AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year.
