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Recombinant Human CXCL9/MIG

Molecule:

CXCL9

Synonyms:

Small inducible cytokine B9, CXCL9, Gamma interferon-induced monokine, chemokine (C-X-C motif) ligand 9, CMK, Humig, SCYB9, crg-10, monokine induced by gamma-interferon

Species:

Human

Cat. No.:

CRMX00C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,700.00 BUY

Description:

Recombinant Human CXCL9/MIG is a single non-glycosylated polypeptide chain containing 103 amino acids.

Background: CXCL9, a member of the α subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine activated gene in mouse macrophages. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The human CXCL9 cDNA encodes a 125 amino acid residue precursor protein with a 22 amino acid residue signal peptide that is cleaved to yield a 103 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has recently been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

Entrez Symbol:

CXCL9

Uniprot Name:

C-X-C motif chemokine 9

Uniprot ID:

Q07325

Data PDF:

CRMX00

Source:

E. coli

Molecular Weight:

~11.7 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4 + 50 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-100 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rHuMIG/CXCL9 as determined by LAL method.

Amino Acid Sequence:

TPVVRKGRCS CISTNQGTIH LQSLKDLKQF APSPSCEKIE IIATLKNGVQ TCLNPDSADVKELIKKWEKQ VSQKKKQKNG KKHQKKKVLK VRKSQRSRQK KTT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Stable at 2-8°C, but best kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at 2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeated freeze/thaw cycles.