Recombinant Human CCN family member 1 / Protein Cyr61

Synonyms:

CCN family member 1, IGF-binding protein 10, IGFBP-10, Protein CYR61

Cat. No.:

CRC023A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$190.00 BUY

Description:

Cysteine-rich angiogenic inducer 61 (CCN1) encoded by the Cyr61 gene is a dynamically expressed, multifunctional matricellular protein and it is also a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family. CCN1 plays essential roles in cardiovascular development during embryogenesis and regulates inflammation, wound healing and fibrogenesis in the adult. Aberrant CCN1 expression is associated with myriad pathologies, including various cancers and diseases associated with chronic inflammation. Mature human CCN1 shares 93 % amino acid sequence identity with mouse and rat CCN1 and consists of four domains. There are an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain.

Entrez Symbol:

CCN1

Uniprot Name:

CCN family member 1

Uniprot ID:

O00622

Data PDF:

CRC023

Molecular Weight:

~39.4 kDa (357 aa) monomer

Formulation:

Lyophilized from a sterile-filtered citrate buffer containing 300 mM NaCl, pH 3.0.

Biological Activity:

ED50 < 3.0 μg/ml, determined by a cell proliferation assay using murine Balb/3T3.

Specific Activity:

>330 IU/mg

Endotoxin Level:

<1 EU/µg as determined by LAL method.

Amino Acid Sequence:

TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVT GQCCEEWVCD EDSIKDPMED QDGLLGKELG FDASEVELTR NNELIAVGKG SSLKRLPVFG MEPRILYNPL QGQKCIVQTT SWSQCSKTCG TGISTRVTND NPECRLVKET RICEVRPCGQ PVYSSLKKGK KCSKTKKSPE PVRFTYAGCL SVKKYRPKYC GSCVDGRCCT PQLTRTVKMR FRCEDGETFS KNVMMIQSCK CNYNCPHANE AAFPFYRLFN DIHKFRD

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Storage/Stability:

Store as supplied for up to 1 year at -20°C to -80°C. Reconstituted protein is stable for one month at 2-8°C. For longer storage, prepare working aliquots and store at -20°C to -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.