Recombinant Human Deoxyribonuclease I

Synonyms:

Deoxyribonuclease I, DNase I, DNL1, DRNI, DNASE1

Cat. No.:

CSI10719 pdf (datasheet)

Quantity/Size:

1500 U

Price:

$450.00 BUY

Description:

Recombinant Human Deoxyribonuclease I is an enzyme that selectively cleaves DNA. It is an endonuclease enzyme that splits phosphodiester linkages within polynucleotides, acting on single stranded DNA, double stranded DNA, and chromatin. DNase is activated by bivalent metals such as Mg2+ and Ca2+.

DNase enzymes are common reagents used in biochemical methods requiring digestion of DNA and recovery of RNA, or where DNAneeds to be removed without affecting structural proteins or enzymes. DNase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membranebound DNA fragments.

Concentration:

1 mg/mL

Data PDF:

CSI10718

Source:

CHO cells

Molecular Weight:

37.0 kDa (260 aa)

Physical State:

Liquid

Formulation:

Sterile filtered, 0.015% Calcium chloride, 0.88% Sodium chloride.

Specific Activity:

1000 IU/mg
DNase activity is assayed according to the photometric method of Kunitz. One Kunitz unit results in an increase in absorbance at 260 nm of 0.001/minute at 25°C when acting upon highly polymerized solution of DNA at pH 5. In addition, 0.

Storage/Stability:

Stable for 2 years at 2-8°C and for 3 weeks at 15°C, pH 6.3.