Recombinant Human EGF, Endotoxin Free

Synonyms:

Pro-epidermal growth factor, Urogastrone

Cat. No.:

CRO507A pdf (datasheet)

Quantity/Size:

10 ug

Price:

$175.00 BUY

Description:

Epidermal Growth Factor (EGF) is a potent growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation. It stimulates the proliferation of various epidermal and epithelial cells. EGF has been shown to inhibit gastric secretion, promote colony formation of epidermal cells in culture and to be involved in wound healing. EGF is the prototype of a large family of EGF-like proteins, it signals through the c-erbB receptor. Recombinant human EGF is a globular protein with 3 intramolecular disulfide-bonds essential for biological activity. Approximately 70% homology is found between human EGF and EGF isolated from other species. The relative positions of the cysteine residues is conserved.

Uniprot ID:

P01133

Data PDF:

CRO507

Source:

Barley

Molecular Weight:

Recombinant human EGF contains 53 aa and an N-terminal 6 aa His-tag for a predicted MW of 7 kDa, but migrates with and apparent MW of 9.5 kDa in SDS-PAGE

Formulation:

Lyophilized from a sterile solution of PBS, pH 7.2

Purity:

> 95% by SDS-PAGE gel analysis

Biological Activity:

ED50 < 0.1 ng/ml, determined by the dose dependent proliferation of 3T3 cells.

Specific Activity:

> 1.0 x 10E-7 U/mg