Recombinant Human EGFR GST Active

Synonyms:

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Species:

Human

Cat. No.:

CSI20284B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$495.00 BUY

Description:

Recombinant human EGFR (668-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity. Binding of epidermal growth factor to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility, and cell survival. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa, and its expression is upregulated in colon cancers and most neoplasms. Activation of EGFR triggers activation of the ERKsignaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation.

Concentration:

0.1 mg/ml

Uniprot Name:

Epidermal growth factor receptor

Uniprot ID:

P00533

Data PDF:

CSI20284

Source:

Sf9 insect cells

Molecular Weight:

Approximately 89-100 kDa

Tag:

N-terminal GST

Formulation:

50 mM Tris-HCl, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% Glycerol.

Purity:

≥ 75% by SDS-PAGE

Specific Activity:

60 nmol/min/mg

Storage/Stability:

Upon receipt, store product at -80°C. Recommended storage upon initial thaw, centrifuge vial to condense liquid, prepare working aliquots (> 20 ml) and store at -80°C. Avoid repeated freeze-thaw cycles.

Application:

WB