Recombinant Human EPO alpha

Molecule:

EPO

Synonyms:

EPO, Epoetin

Species:

Human

Cat. No.:

CRE600A pdf (datasheet)

Quantity/Size:

500 U

Price:

$230.00 BUY

Description:

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. Human EPO is processed from a 193 amino acid precursor protein to yield the mature, active protein that contains 166 amino acids. EPO contains one O-linked and three N-linked sites of glycosylation, which is required for biological activity in vivo.

CRE600 (liquid) is IDENTICAL to CRE134 (lyophilized)

Concentration:

Lot specific, typically 140 IU/ml

Entrez Symbol:

EPO

Uniprot Name:

Erythropoietin

Uniprot ID:

P01588

Data PDF:

CRE600

Source:

CHO cells

Molecular Weight:

Predicted, approximately 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE.

Physical State:

Liquid

Formulation:

Sterile filtered sodium citrate buffer (1 liter of ddH2O containing 5.9 g of sodium citrate, 5.8 g of sodium chloride and 0.06 g of citric acid).

Purity:

>98% by SDS-PAGE and HPLC analyses

Biological Activity:

Fully biologically active when compared to standard. The Specific Activity was measured by the stimulation of reticulocyte production in normocythaemic mice and was determined to be > 1.5 × 10e5 IU/mg .

Specific Activity:

> 1.5 × 10e5 IU/mg .

Endotoxin Level:

<0.01 EU/μg by LAL method

Amino Acid Sequence:

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

Storage/Stability:

Store at 2-8 oC for up to 6 months.