Recombinant Human EPO alpha

Molecule:

EPO

Synonyms:

Erythropoietin, Epoietin

Species:

Human

Cat. No.:

CRE134A pdf (datasheet)

Quantity/Size:

500 U

Price:

$230.00 BUY

Description:

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. Human EPO is processed from a 193 amino acid precursor protein to yield the mature, active protein that contains 166 amino acids. EPO contains one O-linked and three N-linked sites of glycosylation, which is required for biological activity in vivo.

CRE134 (lyophilized) is IDENTICAL to CRE600 (liquid)

Entrez Symbol:

EPO

Uniprot Name:

Erythropoietin

Uniprot ID:

P01588

Data PDF:

CRE134

Source:

CHO cells

Molecular Weight:

Predicted, approximately 21 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 36-40 kDa in SDS-PAGE.

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2μm filtered concentrated solution in sodium citrate buffer.

Purity:

>98% by SDS-PAGE and HPLC analyses

Biological Activity:

Lot specific, typically 140 IU/ul prior to lyophilization

Specific Activity:

≥ 1.5 × 105 IU/mg, determined by stimulation of reticulocyte production in normocythaemic mice.

Endotoxin Level:

< 0.01EU/μg of rHuEPO-α as determined by LAL method

Amino Acid Sequence:

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

Reconstitution:

Centrifuge vial prior to opening. Reconstitute with sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. . Further dilutions should be made in appropriate buffered solution.

Storage/Stability:

This lyophilized product is stable, as supplied for up to 1 year at -20°C to -80°C. Upon reconstitution under sterile conditions, the preparation is stable for up to 1 month at 2-8° C. For maximal stability, prepare working aliquots under sterile conditions and store at -20°C to -80°C or up to 3 months. Avoid repeated freeze/thaw cycles.