Recombinant Human Fas Ligand, soluble, His-tag

Synonyms:

Tumor necrosis factor ligand superfamily member 6, TNFSF6, FASL, FASLG, CD178, CD95 ligand, CD95L

Species:

Human

Cat. No.:

CRF156B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$520.00 BUY

Description:

Fas Ligand is a member of the TNF superfamily that is expressed on the cell surface of activated T cells. Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. Both human and murine sFasL are fully active on human and murine cells. Recombinant human soluble Fas Ligand comprises the TNF homologous region of FasL plus an eight His-Tag.

Entrez Symbol:

FASLG

Uniprot Name:

Tumor necrosis factor ligand superfamily member 6

Uniprot ID:

P48023

Data PDF:

CRF156

Source:

CHO cells

Molecular Weight:

17.9 kDa (156 aa)

Tag:

His

Formulation:

Lyophilized without additives

Purity:

≥ 95% by RP-HPLC and SDS-PAGE

Biological Activity:

ED50 ≤ 10.0 ng/ml, determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking.

Specific Activity:

≥ 1 x 10e5 units/mg

Endotoxin Level:

< 1 EU/µg

Amino Acid Sequence:

HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL

Storage/Stability:

Stable for 1 year at -20°C to -80°C.