Recombinant Human FAS Receptor / CD95

Molecule:

Fas/CD95

Synonyms:

FAS, CD95, Tumor necrosis factor receptor superfamily member 6, TNFRSF6, FASLG receptor, Apoptosis-mediating surface antigen RAS, Apo-1 antigen, FAS Receptor

Species:

Human

Cat. No.:

CRC804B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$520.00 BUY

Description:

Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fasbearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide corresponding to the TNFR homologous cysteine rich extracellular domain Fas.

Entrez Symbol:

FAS

Uniprot ID:

P25445

Data PDF:

CRC804B

Source:

E. coli

Molecular Weight:

17.6 kDa (157 aa)

Formulation:

Lyophilized without additives

Purity:

> 95% as determined by SDS-PAGE and HPLC analyses

Biological Activity:

ED50 = 10-15 µg/ml, determined by its ability to inhibit the cytotoxicity of Jurkat cells in the presence of 2 ng/ml of hFasL

Endotoxin Level:

< 1 EU/µg

Amino Acid Sequence:

MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS

Storage/Stability:

Stable at -20°C to -80° for up to 1 year.