Description:
Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Human FGF-22 is a member of the FGF-7 subfamily and is synthesized by multiple cell lines including neurons, keratinocytes and skeletal muscle myotubes. Human FGF-22 shares 86% homology with mouse FGF-22. Recombinant human FGF-22 is a non-glycosylated protein, containing149 amino acids.
Uniprot Name:
Fibroblast growth factor 22
Molecular Weight:
17.3 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution without additives.
Purity:
≥ 97% as determined by HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm.
Biological Activity:
The activity, as determined by the dose-dependent proliferation of 4MBr-5 cells, is typically 50-300 ng/ml.
Endotoxin Level:
Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.
Amino Acid Sequence:
MTPSASRGPR SYPHLEGDVR WRRLFSSTHF FLRVDPGGRV QGTRWRHGQD SILEIRSVHV GVVVIKAVSS GFYVAMNRRG RLYGSRLYTV DCRFRERIEE NGHNTYASQR WRRRGQPMFL ALDRRGGPRP GGRTRRYHLS AHFLPVLVS
Reconstitution:
Centrifuge vial prior to opening. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Storage/Stability:
Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage.
