Synonyms:
HBGF-5, Smag-82, heparin-binding growth factor 5, FGF-5
Description:
Background: Fibroblast Growth Factor-5 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. Product: Recombinant Human FGF-5 is a 27.5kD protein containing 252 amino residues.
Uniprot Name:
fibroblast growth factor 5
Molecular Weight:
27.5 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution without additives.
Purity:
>95% by SDS-PAGE and HPLC analyses.
Biological Activity:
ED50: 0.5-1.0 ng/ml (As determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors). For most in vitro applications, FGF-5 exerts its biological activity in the concentration range of 0.1 to 20.0 ng/ml.
Endotoxin Level:
<0.1 ng/�g
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in 1 X PBS, pH 7.2 to a concentration of 0.5-1.0 mg/ml. The solution can then be further diluted in other aqueous buffers.
Storage/Stability:
Lyophilized protein is stable at room temperature, but is preferably stored desiccated at<0℃C. After reconstitution, aliquot and store at -20℃C. Avoid repeated freeze-thaw cycles.
