Synonyms:
FGF-6, Heparin secretory-transforming protein 2, HST-2, HSTF-2, Heparin-bindinggrowth factor 6, HBGF-6
Description:
Fibroblast growth factor 6 (FGF-6) is a heparin-binding growth factor that is expressed in
epithelial and mesenchymal lineages. FGF-6 binds and signals through the FGF
receptors FGFR1, FGFR2, and FGFR4. FGF-6 functions as a mitogen for vascular
endothelial cells and fibroblasts. Plays an important role in the regulation of cell
proliferation, cell differentiation, angiogenesis and myogenesis, and is required for normal
muscle regeneration.
Uniprot Name:
Fibroblast growth factor 6
Molecular Weight:
18.9 kDa (169 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from sterile-filtered 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 1.0 ng/ml, determined by its ability to induce the proliferation of mouse 3T3 fibroblasts, in the presence of heparin.
Specific Activity:
≥ 1.0 x 10e6 units/mg.
Endotoxin Level:
≤ 1 EUs/µg by kinetic LAL analysis
Amino Acid Sequence:
MGTRANNTLL DSRGWGTLLS RSRAGLAGEI AGVNWESGYL VGIKRQRRLY CNVGIGFHLQ VLPDGRISGT HEENPYSLLE ISTVERGVVS LFGVRSALFV AMNSKGRLYA TPSFQEECKF RETLLPNNYN AYESDLYQGT YIALSKYGRV KRGSKVSPIM TVTHFLPRI
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
