Recombinant Human FGF21

Molecule:

FGF-21

Synonyms:

FGF-21, FGFL

Species:

Human

Cat. No.:

CRF159C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$2,720.00 / Special Price: $2,220.00 BUY

Description:

Fibroblast growth factor-21 (FGF-21) is a member of the FGF family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. FGF-21 is a secreted endocrine factor that functions as a major metabolic regulator. FGF-21 stimulates the uptake of glucose in adipose tissue. Activity requires the presence of beta-klotho (KLB)

Entrez Symbol:

FGF21

Uniprot Name:

Fibroblast growth factor 21

Uniprot ID:

Q9NSA1

Data PDF:

CRF159

Source:

E. coli

Molecular Weight:

19.5 kDa (182 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 500 ng/ml, determined by proliferation of 3T3 cells with β-klotho and heparin

Specific Activity:

≥ 2.0 x 10e3 unit/mg

Endotoxin Level:

≤ 1 EUs/µg by kinetic LAL analysis.

Amino Acid Sequence:

MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPALPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.