Recombinant Human FLT3 Ligand

Molecule:

FLT3L

Synonyms:

FLT3L, fms related tyrosine kinase 3 ligand

Species:

Human

Cat. No.:

CRF110A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$85.00 BUY

Description:

Flt-3 ligand (Flt-3L) is a hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3L is structurally related to MCSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human Flt-3L isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced Flt-3L mRNA, encoding a soluble form of the human FLT-3L, has also been identified. Flt-3L is widely expressed in various human and mouse tissues. Human FLT-3 ligand is active on mouse cells. Flt-3L has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors. Furthermore, Flt-3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.

Entrez Symbol:

FLT3LG

Uniprot Name:

Fms-related tyrosine kinase 3 ligand

Uniprot ID:

P49771

Data PDF:

CRF110

Source:

E. coli

Molecular Weight:

Monomer, 17.6 kDa (155 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 <10 ng/ml, determined by dose-dependent proliferation of OCI-AML5 cells.

Specific Activity:

≥1 x 105 units/mg

Endotoxin Level:

≤1 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.