Recombinant Human FLT3 Ligand, Endotoxin Free

Molecule:

FLT3LG

Synonyms:

FLT3 Ligand, FL, Flk-2 ligand, FLT3L, Ly72L, Ly72 ligand, STK-1 ligand

Species:

Human

Cat. No.:

CRF133D pdf (datasheet)

Quantity/Size:

100 μg

Price:

$901.00 BUY

Description:

FLT3 Ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor and colony stimulating factor 1. In synergy with other growth factors and interleukins, FLT3 Ligand stimulates the proliferation and differentiation of early hematoepoietic cells. It is a major growth factor stimulating the growth of dendritic cells. Recombinant human FLT3L contains 156 amino acids and a 16aa His-tag.

Uniprot ID:

P49771

Data PDF:

CRF133

Source:

Barley

Molecular Weight:

The predicted molecular weight of the recombinant FLT3 Ligand is 19.9 kDa, but the recombinant protein migrates as two bands with an apparent molecular mass of 20 and 22 kDa in SDS-PAGE as a result of glycosylation.

Formulation:

Lyophilized from a 0.2 µm sterile filtered solution of PBS, pH 7.2

Purity:

>95% by SDS-PAGE. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF alpha and IL-1beta induction.

Biological Activity:

ED50< 0.3 ng/ml, determined by dose-dependent proliferation of recombinant human FLT3 Ligand on AML5 cells.

Specific Activity:

>3.3 x 10E-6 units/mg

Reconstitution:

Centrifuge vial before opening. Reconstitute the lyophilized protein using sterile distilled water to a concentration no less than 0.1 mg/ml.

Storage/Stability:

Store lyophilized protein at -20°C and reconstituted protein in working aliquots at -20°C with a carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage.