Recombinant Human Follistatin

Synonyms:

FST, FS, Activin-binding protein.

Species:

Human

Cat. No.:

CRF024B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$185.00 BUY

Description:

Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors.

Entrez Symbol:

FST

Uniprot Name:

Follistatin

Uniprot ID:

P19883

Source:

E. coli

Molecular Weight:

31.7 kDa (289 aa)

Formulation:

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Purity:

> 95.0% by reducing and non-reducing SDS-PAGE

Biological Activity:

≤ 200 ng/ml, determined by the dose-dependent neutralization of Human Activin A induced cytotoxicity of MPC-11 cells

Specific Activity:

≥ 5.0 x 103units/mg

Endotoxin Level:

≤ 1 EU/μg, by kinetic LAL analysis

Amino Acid Sequence:

MGNCWLRQAK NGRCQVLYKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DFKVGRGRCS LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCN

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.