Recombinant Human Follistatin

Synonyms:

FST, FS, Activin-binding protein.

Species:

Human

Cat. No.:

CRF024C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$5,680.00 / Special Price: $3,720.00 BUY

Description:

Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors.

Recombinant human Follistatin is a non-glycosylated protein, containing 289 amino acids.

Amino Acid Sequence: MGNCWLRQAK NGRCQVLYKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DFKVGRGRCS LCDELCPDSK SDEPVCASDN ATYASECAMK EAACSSGVLL EVKHSGSCN

Entrez Symbol:

FST

Data PDF:

CRF024

Source:

E. coli

Molecular Weight:

31.7 kDa

Formulation:

Recombinant human Follistatin is lyophilized from 10 mM Na2PO4, pH 7.35 and 50 mM NaH2C6H5O7 (sodium citrate).

Purity:

Greater than 95.0% as determined by: Reducing and Non-reducing SDS-PAGE.

Biological Activity:

The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.

Endotoxin Level:

Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein.

Reconstitution:

When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL

Storage/Stability:

Lyophilized product is very stable at -20°C. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.