Recombinant Human Gastric Intrinsic Factor

Synonyms:

Cobalamin binding intrinsic factor, Intrinsic factor, INF, IF

Species:

Human

Cat. No.:

CRI105B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$300.00 BUY

Description:

Intrinsic Factor is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12 in the terminal ileum. Vitamin B12 is essential for erythrocyte maturation and mutations in the Intrinsic Factor may lead to congenital pernicious anemia. Upon entry into the stomach, vitamin B12 binds to one of two B12 binding proteins present in the gastric fluid. In the less acidic environment of the small intestine, these proteins dissociate from the vitamin, allowing it to bind to intrinsic factor and enter the portal circulation through a receptor in the ileal mucosa specific for the B12-intrinsic factor complex.

The Intrinsic Factor is fused to a hexa-histidine at the C-terminus.

Concentration:

> 0.5 mg/ml

Uniprot Name:

Cobalamin binding intrinsic factor

Uniprot ID:

P27352

Data PDF:

CRI105

Source:

Sf9 insect cells

Molecular Weight:

55 kDa

Formulation:

Sterile-filtered solution containing 20 mM HEPES, 100 mM NaCl, pH 8.0, with 20% Glycerol.

Purity:

> 95.0% by RP-HPLC and SDS-PAGE analyses.
Purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.

Storage/Stability:

Upon receipt, store at 2-8°C if entire vial will be used within 2-4 weeks, otherwise store working aliquots at -20°C to -80°C. It is recommended to add a carrier protein, such as 0.1% HSA or BSA to the solution before freezing aliquots. Avoid repeated freeze-thaw cycles.