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Recombinant Human GCSF Animal Free

Molecule:

CSF3

Synonyms:

CSF3OS, GCSF, granulocyte colony-stimulating factor, filgrastim, lenograstim, pluripoietin

Species:

Human

Cat. No.:

CRG300C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$3,870.00 / Special Price: $2,980.00 BUY

Description:

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-a and IFN-g. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-a activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively. Human and mouse GCSF are cross-reactive. Recombinant Human Granulocyte-Colony Stimulating Factor is a single non-glycosylated polypeptide chain containing 175 amino acids.

Manufactured without Animal-derived products, in an Animal Free facility.

Uniprot ID:

P09919

Data PDF:

CRG300-AF

Source:

E. coli

Molecular Weight:

18.8 kDa

Physical State:

Lyophilized

Formulation:

Sterile-filtered aqueous solution containing 20 mM acetic acid, 50 mM NaCl.

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤60 pg/ml, determined by dose-dependent proliferation by NFS-60 cells.

Specific Activity:

> 1.7 x 107 U/mg The specific activity of Human G-CSF is approximately 3.9 x 10^4 IU/μg, which is calibrated against recombinant human G-CSF WHO International Standard (NIBSC code: 09/136).

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.