Synonyms:
GDNF, ATF1, ATF2, HSCR3, HFB1-GDNF
Description:
Recombinant Human GDNF is a non-glycosylated homodimer, containing two 135 amino acid chains.
Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a neurotrophic factor that is closely related to other neurotrophic factors, such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes RET and GFRα1-4, to promote dopamine uptake, survival and differentiation of neurons.
Uniprot Name:
Glial cell line-derived neurotrophic factor
Genbank Accession No:
P39905
Molecular Weight:
30.4 kDa (monomer 15.2 kDa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution in 10 mM Sodium Citrate + 100 mM NaCl, pH 4.0
Purity:
>95% as determined by SDS-PAGE
Biological Activity:
The biological activity as determined by the dose-dependent proliferation of C6 cells is typically 0.5-5 μg/mL.
Specific Activity:
2 x 10E3 units/mg
Endotoxin Level:
<1 EU/µg of protein (50% confidence range).
Amino Acid Sequence:
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Lyophilized protein is stable at room temperature for shipping purposes. Store desiccated at -20°C on receipt. After reconstitution, stable for ≤1 month at 2-8°C. For long term, store aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
