Synonyms:
Glial cell line-derived Neurotrophic Factor, ATF1, ATF2, HFB1-GDNF
Description:
Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a neurotrophic factor that is closely related to other neurotrophic factors, such as Neurturin, Persephin, and Artemin, by a common structural feature called the cysteine-knot. GDNF signals through a multicomponent system of receptors that includes RET and GFRα1-4, to promote dopamine uptake, survival and differentiation of neurons.
Uniprot Name:
Glial cell line-derived neurotrophic factor
Molecular Weight:
Homodimer, 15.2/30.4 kDa (135/270 aa)
Formulation:
Sterile filtered solution in 10 mM sodium citrate, 100 mM Sodium Chloride, pH 4.0
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 3.0 µg/ml, determined by the dose-dependent proliferation of C6 cells
Specific Activity:
≥ 3.3 x 102 U/mg
Endotoxin Level:
≤ 1 EU/µg by kinetic LAL
Amino Acid Sequence:
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Upon receipt, store as supplied at -20°C to -80°C for up to one year. Upon reconstitution, the preparation is stable for up to 1 month at 2-8 °C, 3 months at -20°C to -80°C. For long term storage dilute to working aliquots containing 0.1% BSA and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
