Recombinant Human GDNF, Endotoxin Free

Synonyms:

ATF-1, ATF, astrocyte-derived trophic factor

Cat. No.:

CRO505A pdf (datasheet)

Quantity/Size:

10 ug

Price:

$325.00 BUY

Description:

Glial cell line derived neurotrophic factor (GDNF) is a protein promoting the survival of various neuronal subpopulations at different stages of development. These include dopaminergic neurons in midbrain, GDNF stimulates their survival, differentiation and high-affinity dopamine uptake. The use of GDNF in the treatment of Parkinson's disease and spinal cord injuries has shown promising results. GDNF belongs to the TGF-ß superfamily, it is a homodimeric protein with the familial cysteine-knot structure. Cells expressing GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons.

Uniprot ID:

P39905

Data PDF:

CRO505

Source:

Barley

Molecular Weight:

Recombinant human GNDF contains 134 aa with 16 aa His-tag, having a predicted MW of 17.2 kDa including the His-tag. As a result of glycosylation rhGNDF migrates with an apparent MW of 25 kDa.

Formulation:

Lyophilized from PBS, pH 7.2, sterile filtered through a 0.2 µm filter.

Purity:

> 95% by SDS-PAGE gel analysis.

Biological Activity:

ED50 < 8.0 ng/ml, determined by the dose-dependent effect of rhGNDF on proliferation of rat SH-SY5Y human neuroblastoma cell line.

Specific Activity:

> 1.3 x 10E-5 U/mg