Recombinant Human GM-CSF Animal Free

Synonyms:

GMCSF, CSF, molgramostin, sargramostim

Cat. No.:

CRG100C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$3,640.00 / Special Price: $2,380.00 BUY

Description:

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. GM-CSF is a growth factor for granulocyte-macrophage, erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells.

Manufactured without Animal-derived products, in an Animal Free facility.

Uniprot ID:

P04141

Data PDF:

CRG100-AF

Source:

E. coli

Molecular Weight:

Monomer, 14.6 kDa (128 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered solution in 10 mM sodium phosphate, pH 7.5.

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤200 pg/ml, as determined by dose-dependent proliferation of human TF-1 cells.

Specific Activity:

1.6 x 10e4 IU/mg, which is calibrated against recombinant human GM-CSF WHO International Standard (NIBSC code: 88/646).

Endotoxin Level:

≤1 EU/µg by kinetic LAL

Amino Acid Sequence:

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.