Recombinant Human GTPase HRas, G12V mutation, GST-tag

Molecule:

HRAS

Synonyms:

Harvey rat sarcoma viral oncogene homolog, H-Ras-1, Transforming protein p21, p21ras

Species:

Human

Cat. No.:

CSI11667 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$430.00 BUY

Description:

Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras-4A, and K-Ras-4B). The mutation G12V leads to elimination of the intrinsic GTPase activity. H-Ras (G12V) is effective in activating PI3K and PKB, whereas N-Ras and K-Ras are more potent towards MAP kinase.

The GST-Tag facilitates the protein’s application in typical GST pull-down assays.

Entrez Symbol:

HRAS

Uniprot Name:

GTPase HRas

Uniprot ID:

P01112

Data PDF:

CSI11667

Source:

E. coli

Formulation:

25 mM Tris-HCl, 150 mM NaCl, 5 mM MgCl2, and 5 mM ß-mercaptoethanol, pH 7.5

Purity:

> 95% by SDS-PAGE

Storage/Stability:

Store at -80°C for up to 1 year. Upon initial thaw, prepare aliquots and store at -80°C. Avoid freeze / thaw cycles.