Recombinant Orf Virus VEGF-E / Heparin-binding Human VEGF165 Chimera

Molecule:

VEGF

Synonyms:

VEGFE, Vascular Endothelial Growth Factor-E, s VEGF-E

Species:

Orf Virus

Cat. No.:

CRV012A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$320.00 BUY

Description:

A DNA sequence encoding the first 116 amino acid residue of Orf virus VEGF-E isolate D1701 (Dehio et al., 1999 EMBO J. 18:363-374; GenBank accession No. AF106020) was fused with a DNA sequence encoding to the C-terminal heparin binding domain of human VEGF165. The chimeric protein was expressed in insect cells using a baculovirus expression system. Based on sequence similarity to VEGF-A, a gene encoding a VEGF homologue has recently been discovered in the genome of Orf virus (OV) (Lyttle et al., 1994 J. Virol 68:84-92). Different isolates of orf virus show significant amino acid sequence similarity to VEGF-A and described as a viral virulence factor that appear to be derived from captured host genes. All eight cysteine residues of the central cysteine knot motif characteristic of members of the VEGF family are conserved among other residues in the VEGF-E proteins (Dehio et al., 1999 EMBO J. 18:363-374; Wise et al., 1999 Proc. Natl. Acad. Sci USA 96:3071-3076). Alignment of all mammalian VEGF sequences indicated that VEGF-E is distinct from the previously described VEGFs but most closely related to VEGF-A. Like VEGF-A, VEGF-E was found to bind with high affinity to VEGF receptor-2 (KDR) resulting in receptor autophosphorylation, whilst in contrast to VEGF-A, VEGF-E and hb-VEGF-E can not bind to VEGF receptor-1 (Flt-1). Therefore VEGF-E is a potent angiogenic factor selectively binding to VEGF receptor–2/ KDR. Compared to human VEGF165 this virus form has no heparin-binding domain and seems to be a freely secreted protein comparable to VEGF121. In order to compare this form with human VEGF165, an additional heparin-binding domain was engineered at the C-terminus to allow interaction with proteo-aminoglycans and heparan sulfate. These form is also able to interact with neuropillin–1.

Uniprot ID:

Q9YMF3

Data PDF:

CRV012

Source:

Insect cells

Molecular Weight:

44 kDa (154 aa)

Formulation:

Lyophilized from 50 mM acetic acid

Purity:

> 90% by SDS-PAGE, visualized by silver stain.

Biological Activity:

ED50 typically 5-20 ng/ml, determined by a cell proliferation assay using primary human endothelial cells (HUVEC).

Endotoxin Level:

< 1 EU/µg

Amino Acid Sequence:

DSTKTWSEVFENSGCKPRPMVFRVHDEHPELTSQRFNPPCVTLMRCGGCCNDESLEC VPTEEANVTMQLMGASVSGGNGMQHLSFVEHKKCDCKPPRDRARQENPCGPCSERR KHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR

Storage/Stability:

Stable at -20°C to -80° for up to 1 year.