IFN-alpha

IFNA, IFA2, IFNA2B, IFN-alphaA

Human

CRI030A pdf (datasheet)

20 μg

$190.00 BUY

Recombinant Human IFN-alpha 2b is a single polypeptide chain containing 165 amino acids.

Background: IFN-alphas are proteins secreted by leukocytes. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

IFNA2

CRI030

Saccharomyces cerevisiae

~19.3 kDa

Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4, with 0.02 % Tween-20.

>98% by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.6 × 10⁸ IU/mg.

Less than 1EU/μg as determined by LAL method.

CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.