Recombinant Human Interferon-alpha 2b

Molecule:

IFN-alpha 2b

Synonyms:

IFNA2b, IFNA, INFA2

Species:

Human

Cat. No.:

CRI004A pdf (datasheet)

Quantity/Size:

20 μg

Price:

$85.00 BUY

Description:

Interferon-alpha 2b (IFN-a2b) is a type I interferon made by leukocytes during viral infection. The JAK-STAT pathway mediates the antiviral and anti-cell proliferation activities of IFN-a2b. IFN-a proteins are widely used as standard treatments during antiviral and antineoplastic therapies. The IFN-a2b variant differs from IFN-a2a by one amino acid.

Entrez Symbol:

IFNA2

Uniprot Name:

Interferon alpha-2

Uniprot ID:

P01563 (VAR_004012)

Data PDF:

CRI004

Source:

E. coli

Molecular Weight:

19.4 kDa (166aa) monomer

Formulation:

Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Activity determined by viral cytopathic effect (CPE) assay using EMC virus on A549 cells.

Specific Activity:

≥ 2.0 x 108 U/mg

Endotoxin Level:

≤ 1 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE

Reconstitution:

Centrifuge vial prior to opening. Add sterile water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipet solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilution should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.